(Inglés version, the monkey clik).
also points to some key ways that could explain why the retrovirus "was not seen" in some previous studies. It is certainly a factor and study of great interest to researchers and sufferer.
XMRV infection of Rhesus macaques February 17, 2011
The first detailed study of nonhuman primates infected with the retrovirus XMRV reveals that the virus establishes a persistent infection characterized by multiple tissue infection
. Viremia (virus in the blood) is low and transient, with detectable proviral DNA in blood lymphocytes. The results show that the rhesus monkey can be used to study XMRV infection, transmission, vaccines and antiviral drugs.
The purpose of this study,
the rhesus monkey (Macaca mulatta), was selected due to its proximity to humans and an immune system similar
. The monkeys used do not have antibodies against the p30 capsid protein of XMRV, indicating that they were not previously infected
. The animals were inoculated intravenously with 3.6 million TCID50 of purified XMRV - a lot of viruses, to ensure the infection the virus used, VP62, was produced by transfection of cells with cloned viral DNA isolated from prostate human.
Virus in the fraction of blood plasma was assayed by quantitative RT-PCR.
Of the three animals infected, the virus was detected in one animal on day 4 and no later than 14 days , and in a second animal from days 14-20. The third animal did not develop detectable viremia proviral DNA was found in peripheral blood mononuclear cells (PBMC) of three monkeys for 3-4 weeks, indicating a successful infection. In a month after infection and proviral DNA was not detectable
. Plasma virus was detected again in one of the reactors on day 291, 16 days after immunization with a mixture of XMRV proteins. This means that
viral DNA was present in this animal, but was not detected
.
XMRV was detected in CD4 + and CD8 + T cells and NK cells but not in B cells and monocytes .
rhesus macaques infected with XMRV not show obvious clinical symptoms. peripheral blood analysis revealed an increased number of circulating B and NK cells . titles viral antibodies were detected after infection and reinfection of animals, but soon declined. Other infected animals were sacrificed during the acute phase of infection to identify the pathological changes and virus replication sites. pathogenic effects not observed, except
for the formation of germinal centers in spleen and lymphoid organs , the expected changes after immune stimulation
The Virus was detected in a wide variety of tissues
,
including spleen, lymph nodes, the lining of the gastrointestinal tract, prostate, testes, cervix, vagina, and pancreas
,
but not in others, including brain, heart, kidney and bladder.
Different types of cells were infected in different tissues 
: lymphocytes in lymphoid organs, macrophages in the lung, epithelial or interstitial cells in other organs.
: lymphocytes in lymphoid organs, macrophages in the lung, epithelial or interstitial cells in other organs. 
this virus."
:
• The authors suggest that in rhesus macaques
, XMRV leading causes an acute infection followed by chronic persistent infection . A persistent infection lasting for long periods of time, chronic infection is a persistent infection that is finally approved. Since the monkeys in this study were all slaughtered, it is not possible to determine whether the infection was cleared.
, XMRV leading causes an acute infection followed by chronic persistent infection . A persistent infection lasting for long periods of time, chronic infection is a persistent infection that is finally approved. Since the monkeys in this study were all slaughtered, it is not possible to determine whether the infection was cleared.
•
XMRV The presence of certain blood cells resembles the pattern in a cohort of patients with ME / CFS
• Virus is present in the prostate early in acute infection - XMRV was identified in prostate tumors
• Virus is present in the prostate early in acute infection - XMRV was identified in prostate tumors
• After the acute phase, virus levels are very low , but could have a different outcome in individuals with immune dysfunction • An animal produces the virus after immunization
, results from immune activation
perhaps cycles of virus production • The virus has an initial acute phase followed by reactivation . The authors comment: "While our study has not been associated XMRV infection with pathogenic mechanisms that could lead to
prostate cancer or chronic fatigue syndrome perhaps cycles of virus production • The virus has an initial acute phase followed by reactivation . The authors comment: "While our study has not been associated XMRV infection with pathogenic mechanisms that could lead to
, we believe that such a link, assuming that there would be a temporary far. " • would be informative to determine whether the XMRV is present in some of the same human tissues that were observed infection in rhesus macaques
Because the study involved only a small number of monkeys (8), experiments should be repeated with other animals, and in different laboratories to verify the results.
I also wonder if the choice of intravenous route of inoculation had an effect on the pattern of infection and tropism. It is well known that viral pathogenesis can determine how the virus enters the host. For example, the same virus can replicate in different tissues or different virulence when inoculated in different ways. This question can be readily addressed through the inoculation of rhesus macaques through different pathways.
The study of viral pathogenesis
(the series of events occurring during viral infection in a series)
in animals is essential to understand how viruses cause disease in humans . However, the results of these studies should always be interpreted with caution because what happens in an animal is not always true for a human being
. For example, mere differences in size, metabolism, and development can have important implications in pathogenesis. In interpreting the results of animal studies, we must consider the adage,
. For example, mere differences in size, metabolism, and development can have important implications in pathogenesis. In interpreting the results of animal studies, we must consider the adage,
" mice lie, exaggerate monkeys'.
Onlamoon, N, Dasgupta, J, Sharma, P, Rogers, K, Suppiah, S Rea, J, Molina, RJ, Gaughan, C., Dong, B, Klein E, Qui X, Devar, S, Schochetman G, Hackett, J, Silverman, R, and Villinger , M (2011)., Viral and distribution of responses Antibody infection of Rhesus monkeys exposed to humans gammaretrovirus XMRV Journal of Virology
* This link, along with the article in the Health Blog Wall Street Journal upon him, was released today same (7 hours ago) on the wall
WPI.
ACCESS TO ABSTRACT OF SCIENTIFIC PUBLICATION IN JOURNAL OF VIROLOGY.
Chawaree Chaipan, Kari A. Dilley, Tobias Paprotka, Krista A. Delviks-Frankenberry, J. Narasimhan Venkatachari, Wei-Shau Hu, and Vinay K. Pathak * Posted February 16, 2011 ONLINE.
Reshu Research in Macaques and the results that were seeing (both in macaques and humans) has already been discussed in this blog last year:
September 24, 2010: NEWS! LigaSFC. Platform for Action of those affected by CFS. XMRV presence in Spain: Study Results IrsiCaixa .
November 23, 2010: Looking for a new blood supply test. Several running.
Note 1: This is just
scientific publication journal on what we already knowing these months ago and commentary an EXPERT on the category of Professor Racaniello on this publication. E s a translation of what was published by Professor Rancaniello in Virology Blog
following the online publication of the findings of this study in macaques that and I spoke a few months ago. The photo is tb. your blog (I hope not bother about it). For a better translation, you can check the blog (either through the links above, or clicking on the monkey).
Note 2: If after acute infection the virus is not detected in the blood (except in one monkey), Will it happen the same in the milk?. It is well known that drugs, almost entirely NO pass into breast milk (and the vast majority of them are
compatible with breastfeeding ), why would spend the retrovirus then?.
To find out this is not necessary to kill any monkey , I've said that I have frozen milk másocasiones and more mothers with and without SFC willing to collaborate. If anyone is interested in check, you can simply tell me!. Until recently one of the few situations where breastfeeding was contraindicated, was just another retrovirus, HIV but a year ago this contraindication has been deleted (Tb. in the "rich") to exceed benefits the possible damages. Why should it be different in the case of XMRV?. I remember a few months ago, a mom commented on the wall of WPI that their daughters had tb. SFC EXCEPT one that was born by Caesarean section and was breastfed. She believed that the fact protected her caesarean section. I believe that the protective effect was just breastfeeding. Well. My daughter tb. born by Caesarean section and was breastfed. Here are two good examples to learn the "mystery."
Note 3: If after some time (soon) in monkeys and no XMRV retrovirus is present in the blood, could it be that the cause of "no detection "XMRV human studies pointing?. And if they are looking only for CRP when sabe.-and-take months to apuntando. not detected, could it be that the cause of
"contamination" pointing to the 4 studies published Retrovirology time in a couple of months?.
Note 4: This study confirms what has already been noted, among others, the researchers
IRSI to subsidize the expansion of
Study where did find the XMRV in the English population, but who pointed to a problem in the CD8, CD4,
....) as noted in early December 2010. to know the new Health Minister Leire Pajin that NEED to fund this study. And we need YA. (Not just the sick, if you need the entire population to be exposed all of it). Study where did find the XMRV in the English population, but who pointed to a problem in the CD8, CD4,
While waiting for the much needed funding, who wish to do their bit by providing some (albeit a EURO), you can do:
From Spain : No cta: 2100 0325 41 02001422 05
From Outside Spain: (International Transfers) IBAN: ES58 SWIFT 210003254102001239-93
: CAIX ESBBXXX Concept: STUDY XMRV SFC Beneficiary: Foundation IrsiCaixa Address: Carretera de Canyet s / n 08916 Badalona (Barcelona) Contact: Lourdes Grau: lgrau@irsicaixa.es
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